The data set consists of SeaFlow-based cell abundance, cell size (equivalent spherical diameter), cellular carbon content and total carbon biomass for picophytoplankton populations, namely the cyanobacteria Prochlorococcus, Synechococcus and small-sized Crocosphaera (2-5 um), and picoeukayotes phytoplankton and nanophytoplankton (2-5 μm ESD), collected during oceanographic cruises since 2010 in the North Pacific Ocean. See data description at Scientifc Data: Nature Publishing Group for more information.
Oceanography, Plankton Ecology, Flow Cytometry
Phytoplankton, Ecology, Diversity, Mathematical Modeling
Compute Infrastructure, Software Analysis Tools, Reproducible Research
Flow cytometry, Bacteria, Phytoplankton
Error Estimation, Causal Inference
The SeaFlow instrument is a shipboard underway flow cytometer that provides continuous multi-parameter single particle measurements without the need for clean water. The instrument eliminates the traditional need for a sheath fluid by employing a unique optical system that relies on three photodetectors to create a virtual core in the sample stream within which the properties of particles are accurately measured. In this way, the instrument is able to collect the equivalent of 1 sample every 3 minutes or every 1 km (for a ship moving at 10 knots) from the ship’s flow-through seawater system. See article for more information.”
A size-structured matrix population model to estimate rates of cell division, carbon fixation and carbon loss in phytoplankton measured by flow cytyometry.
A Kernel-based change detection method to map shifts in phytoplankton communities measured by flow cytometry. In this project in collaboration with Dr.
Popcycle is an R package that offers a reproducible approach to process, calibrate and curate flow cytometry data measured by SeaFlow.